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expression construct encoding egfp tagged p4mx1  (Addgene inc)


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    Addgene inc expression construct encoding egfp tagged p4mx1
    ( a ) U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells were fed 3 μm polystyrene microbeads, treated with LLOMe (1 mM, 10 min) as indicated, immunostained for LAMP1 ( red ) and VAPA ( green ), and imaged by DeltaVision microscopy. Scale bar, 10 µm. ( b ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged OSBP ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( c ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged PI4P sensor <t>P4MX1</t> ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( d ) Phase contrast (Phaco) and fluorescence images of polystyrene microbead-containing untreated or LLOMe-treated (1 mM, 10 min) U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells immunostained for OSBP ( magenta ) and ALIX ( green ) or ORP9 ( magenta ) and hIST1 ( green ) and counterstained with DAPI ( blue ). Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( e ) Percentage of microbead-containing lysosomes with positive immunostaining for OSBP or ORP9 in cells treated as in (d). Data are means ± SD of three independent experiments.
    Expression Construct Encoding Egfp Tagged P4mx1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression construct encoding egfp tagged p4mx1/product/Addgene inc
    Average 93 stars, based on 2 article reviews
    expression construct encoding egfp tagged p4mx1 - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair"

    Article Title: VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair

    Journal: bioRxiv

    doi: 10.1101/2025.10.23.684214

    ( a ) U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells were fed 3 μm polystyrene microbeads, treated with LLOMe (1 mM, 10 min) as indicated, immunostained for LAMP1 ( red ) and VAPA ( green ), and imaged by DeltaVision microscopy. Scale bar, 10 µm. ( b ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged OSBP ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( c ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged PI4P sensor P4MX1 ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( d ) Phase contrast (Phaco) and fluorescence images of polystyrene microbead-containing untreated or LLOMe-treated (1 mM, 10 min) U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells immunostained for OSBP ( magenta ) and ALIX ( green ) or ORP9 ( magenta ) and hIST1 ( green ) and counterstained with DAPI ( blue ). Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( e ) Percentage of microbead-containing lysosomes with positive immunostaining for OSBP or ORP9 in cells treated as in (d). Data are means ± SD of three independent experiments.
    Figure Legend Snippet: ( a ) U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells were fed 3 μm polystyrene microbeads, treated with LLOMe (1 mM, 10 min) as indicated, immunostained for LAMP1 ( red ) and VAPA ( green ), and imaged by DeltaVision microscopy. Scale bar, 10 µm. ( b ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged OSBP ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( c ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged PI4P sensor P4MX1 ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( d ) Phase contrast (Phaco) and fluorescence images of polystyrene microbead-containing untreated or LLOMe-treated (1 mM, 10 min) U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells immunostained for OSBP ( magenta ) and ALIX ( green ) or ORP9 ( magenta ) and hIST1 ( green ) and counterstained with DAPI ( blue ). Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( e ) Percentage of microbead-containing lysosomes with positive immunostaining for OSBP or ORP9 in cells treated as in (d). Data are means ± SD of three independent experiments.

    Techniques Used: Microscopy, Labeling, Expressing, Fluorescence, Immunostaining



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    expression construct encoding egfp tagged p4mx1  (Addgene inc)
    93
    Addgene inc expression construct encoding egfp tagged p4mx1
    ( a ) U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells were fed 3 μm polystyrene microbeads, treated with LLOMe (1 mM, 10 min) as indicated, immunostained for LAMP1 ( red ) and VAPA ( green ), and imaged by DeltaVision microscopy. Scale bar, 10 µm. ( b ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged OSBP ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( c ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged PI4P sensor <t>P4MX1</t> ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( d ) Phase contrast (Phaco) and fluorescence images of polystyrene microbead-containing untreated or LLOMe-treated (1 mM, 10 min) U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells immunostained for OSBP ( magenta ) and ALIX ( green ) or ORP9 ( magenta ) and hIST1 ( green ) and counterstained with DAPI ( blue ). Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( e ) Percentage of microbead-containing lysosomes with positive immunostaining for OSBP or ORP9 in cells treated as in (d). Data are means ± SD of three independent experiments.
    Expression Construct Encoding Egfp Tagged P4mx1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression construct encoding egfp tagged p4mx1/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    expression construct encoding egfp tagged p4mx1 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells were fed 3 μm polystyrene microbeads, treated with LLOMe (1 mM, 10 min) as indicated, immunostained for LAMP1 ( red ) and VAPA ( green ), and imaged by DeltaVision microscopy. Scale bar, 10 µm. ( b ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged OSBP ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( c ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged PI4P sensor P4MX1 ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( d ) Phase contrast (Phaco) and fluorescence images of polystyrene microbead-containing untreated or LLOMe-treated (1 mM, 10 min) U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells immunostained for OSBP ( magenta ) and ALIX ( green ) or ORP9 ( magenta ) and hIST1 ( green ) and counterstained with DAPI ( blue ). Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( e ) Percentage of microbead-containing lysosomes with positive immunostaining for OSBP or ORP9 in cells treated as in (d). Data are means ± SD of three independent experiments.

    Journal: bioRxiv

    Article Title: VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair

    doi: 10.1101/2025.10.23.684214

    Figure Lengend Snippet: ( a ) U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells were fed 3 μm polystyrene microbeads, treated with LLOMe (1 mM, 10 min) as indicated, immunostained for LAMP1 ( red ) and VAPA ( green ), and imaged by DeltaVision microscopy. Scale bar, 10 µm. ( b ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1, VPS13C-KO2 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged OSBP ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( c ) Time-lapse images of U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells containing 3 µm pHrodo-labeled microbeads ( magenta ), expressing GFP-tagged PI4P sensor P4MX1 ( green ) and treated with 1 mM LLOMe for the indicated time. Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( d ) Phase contrast (Phaco) and fluorescence images of polystyrene microbead-containing untreated or LLOMe-treated (1 mM, 10 min) U2OS wildtype (WT), VPS13C-KO1 or PI4K2A-KO cells immunostained for OSBP ( magenta ) and ALIX ( green ) or ORP9 ( magenta ) and hIST1 ( green ) and counterstained with DAPI ( blue ). Cells were imaged by spinning disk microscopy. Scale bar, 10 µm. ( e ) Percentage of microbead-containing lysosomes with positive immunostaining for OSBP or ORP9 in cells treated as in (d). Data are means ± SD of three independent experiments.

    Article Snippet: The expression construct encoding eGFP-tagged P4Mx1 (Addgene, 108121) was described in Zewe et al. .

    Techniques: Microscopy, Labeling, Expressing, Fluorescence, Immunostaining